Ewelina Stoczynska-Fidelus, Michal Bienkowski, Sylwester Piaskowski, Mateusz Banaszczyk, Marta Winiecka, Jolanta Zieba, Krystyna Hulas-Bigoszewska, Anna Radomiak-Zaluska, Piotr Rieske

Department of Tumour Biology, Medical University of Lodz, Poland

Abstract:

Nowadays senescence become a widely analyzed mechanism. We observed that majority of  glioblastoma cells became quickly non-proliferative (did not incorporate BrdU)and were β-Gal positive in early passages of cell culture. Standard monolayer conditions positively selected normal, most likely glioma associated stromal cells, versus glioma cells. In passage 2, only single GFAP expressing neoplastic cells were observed. Intriguingly, all of these cells were β-Gal positive, however, they did not show SAHF. Moreover, microarray analyses comparing glioma samples enabling and not-enabling the cell line stabilisation did not show any differences in the senescence markers expression. Thus our results did not allow to determine whether the senescence is the mechanism responsible for glioma cell culture failure. Moreover, they generate the question whether the β-Gal staining is a specific marker precisely identifying senescent cells. It is not clear if SAHF are observed in the senescence of all types of cells (including neoplastic cells) and which markers are present in case of stress- or oncogene-induced senescence of neoplastic cells.

This study was supported by the National Science Centre Grant No. 2011/03/N/NZ1/06534.